heag1 polyclonal antibody (Alomone Labs)
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heag1 polyclonal antibody
Heag1 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heag1+polyclonal+antibody/Anti-KCNH1+(EAG-1)+Antibody/pm22248279-74-12-22
Average 93 stars, based on 9 article reviews
Heag1 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heag1+polyclonal+antibody/Anti-KCNH1+(EAG-1)+Antibody/pm22248279-74-12-22
Average 93 stars, based on 9 article reviews
heag1 polyclonal antibody - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Overexpression of potassium channel ether à go-go in human osteosarcoma."
Article Title: Overexpression of potassium channel ether à go-go in human osteosarcoma.
Journal: Neoplasma
doi: 10.4149/neo_2012_027
Figure Legend Snippet: Figure 2. Determination of the titer of hEAG1 polyclonal antibody by ELISA. (A) The antibody was diluted serially as shown on the top. Positive control was anti-His antibody; Negative control was pre-immunized rabbit serum. (B) Quantization of absorbance values shown in Fig. 2A.
Techniques Used: Enzyme-linked Immunosorbent Assay, Positive Control, Negative Control
Figure Legend Snippet: Figure 4. Analysis of the immunoreactivity of hEAG1 antibodies by Western blot. Mouse brain extracts were prepared, separated on SDS-PAGE and trans- ferred on PVDF membrane as described in Materials. Membranes were immunolabelled with the polyclonal antibody we generated (diluted 1:1000, lane 1), or with the commercial hEAG1 antibody (diluted 1:200, lane 2). The bands revealed by both antibodies had a molecular weight between 150 and 100 kDa. M: marker.
Techniques Used: Western Blot, SDS Page, Membrane, Generated, Molecular Weight, Marker
Figure Legend Snippet: Figure 3. Analysis of the activity and specificity of hEAG1 polyclonal antibody by Western blot. (A) Western blot showing the activity of hEAG1 polyclonal anti- body. Lane 1, lysate of E. coli BL21(DE3) cells harboring pRSET-A- hEAG1F after induction; Lane 2, lysate of HEK 293 cells transfected by pcDNA3.1-hEAG1; Lane 3, lysate of HEK 293 cells transfected by pcDNA3.1(+) empty vector. (B) Western blot showing the specificity of hEAG1 polyclonal antibody. Lysate of HEK293 cells transfected by pcDNA3.1 empty vector, pcDNA3.1-hEAG1 or pcDNA3.1-hEAG2 was detected by the indicated antibodies.
Techniques Used: Activity Assay, Western Blot, Transfection, Plasmid Preparation
Figure Legend Snippet: Figure 5. Immunofluorescence analysis of hEAG1 subcellular localization by hEAG1 polyclonal antibody. HEK293 cells were either mock transfected with pcDNA3.1(+) or transfected with pcDNA3-hEAG1, and then stained with hEAG1 polyclonal antibody. Positive hEAG1 staining was red and DAPI (indicating the nucleus) was blue. The scale bar: 10 μm.
Techniques Used: Transfection, Staining
Figure Legend Snippet: Figure 6. Immunohistochemical staining of hEAG1 in human osteosar- coma samples. hEAG1 immunostaining was detected in a human brain specimen (positive control, A) , 71.4% of human osteosarcoma specimens (B) and (15.8%) osteochondromas specimens (C). Images were captured using an OL- YMPUS light microscope equipped with a CCD colour camera at a 400× magnification. The scale bar: 50 μm.
Techniques Used: Immunohistochemical staining, Staining, Immunostaining, Positive Control, Light Microscopy
Related Articles
Western Blot:Article Title: Overexpression of potassium channel ether à go-go in human osteosarcoma. Article Snippet: The membranes were incubated overnight at 4°C with either hEAG1 polyclonal antibody we generated (1:1000) or hEAG2 antibody (SAB2104244, Sigma, USA) in blocking solution, then with horseradish peroxidase (HRP) conjugated goat anti-mouse antibody (1:10,000) or goat anti-rabbit antibody (1:15,000), and finally developed by enhanced chemiluminescence system. .. Moreover, Western blot analysis was also used to test the immunoreacitivity of Generated:Article Title: Overexpression of potassium channel ether à go-go in human osteosarcoma. Article Snippet: The membranes were incubated overnight at 4°C with either hEAG1 polyclonal antibody we generated (1:1000) or hEAG2 antibody (SAB2104244, Sigma, USA) in blocking solution, then with horseradish peroxidase (HRP) conjugated goat anti-mouse antibody (1:10,000) or goat anti-rabbit antibody (1:15,000), and finally developed by enhanced chemiluminescence system. .. Moreover, Western blot analysis was also used to test the immunoreacitivity of Expressing:Article Title: Overexpression of potassium channel ether à go-go in human osteosarcoma. Article Snippet: The membranes were incubated overnight at 4°C with either hEAG1 polyclonal antibody we generated (1:1000) or hEAG2 antibody (SAB2104244, Sigma, USA) in blocking solution, then with horseradish peroxidase (HRP) conjugated goat anti-mouse antibody (1:10,000) or goat anti-rabbit antibody (1:15,000), and finally developed by enhanced chemiluminescence system. .. Moreover, Western blot analysis was also used to test the immunoreacitivity of |